WWW.WAS.ORG • WORLD AQUACULTURE • JUNE 2013 59 • serum protein level • serum lipid concentration • serum cholesterol concentration • leukocytic formula: white blood cells, eosinophils, stab neutrophils, segmental neutrophils, basophiles, monocytes, lymphocytes and blasts. There is also an opportunity to record the results of histological investigations of liver and gonads, for example, in descriptive form. Such a program allows matching, with high accuracy, individual breeders using their electronic passports and eliminating the probability of mistakes in crossing different lines. Each broodfish has an electronic passport that shows its origin, physiological status and all control research (Fig. 7). The characteristics of sterlet broodstock from the River Don are shown in the Tables 2 and 3. At present, it consists of individuals 3-6 years old. Stable hydrological and hydrochemical conditions of the water environment and effective feeding allow high growth rates of female broodstock. Multiple generations of fry and broodstock have high reproductive characteristics; average fecundity ranged from 15.5 to 36.3 thousand eggs, confirmation of the good quality of sterlet females. Males of the River Don population also have high reproductive indicators. Reproductive characteristics of different generations of males did not differ practically (Table 4, 5). This is the result of the stable regime of environmental factors in the recirculation system. Comparative analysis of formed lines of broodstock of Volga River sterlet indicates high biological performance of females and males of different ages. This was also connected to stable hydrological and hydrochemical conditions of the water environment and optimal feeding of breeders at different stages of ontogenesis. Sterlet eggs were artificially inseminated with thawed, cryopreserved sperm that had been stored in liquid nitrogen for 2 years. This reproductive material was 10 percent of the total number of broodstock that took part in spawning. Before fertilization, the quality of thawed sperm was good, resulting in a fertilization rate of 80 percent, compared to 90 percent in controls. Survival of fry after incubation for 6 days was 75 percent in the control group and 60 percent in the treatment group (Table 6). Morphometric measurements of the larvae of investigated groups were not significantly different. The same results were obtained in trials of fingerlings and fry. Development of starlet broodstock by several methods, including sperm cryopreservation, is reasonable in recirculation systems. It reduces inbreeding and decreases the area required to contain broodstock. Development of sturgeon culture broodstocks and sperm cryobanks allows conservation of the gene pool and decreases the risks of anthropogenic influence on productivity. Notes E.N. Ponomareva and M.M. Belaya, 41 Chekhov Avenue, Rostovon-Don, Russia, 344006 344006, Россия, Ростов-на-Дону, проспект Чехова 41 References Barannikova, I.A., S.I. Nikanorov and A.N. Belousov. 2000. The problem of sturgeon fishes conservation in Russia today. Pages 7-8 In: Sturgeon Fishes at the Turn of the XXI Century. Astrakhan, Russia. Burtzev, I.A. and A.I. Nikolaev. 1999. Methods of formation and exploitation of sturgeon broodstocks in conditions of sturgeon fish hatcheries to purposes of pasturable mariculture. Pages 20-21 In: Resource-Saving Technologies in Aquaculture. Krasnodar. Russia. Podushka, S.B. 1997. Formation and exploitation of sturgeon fish’s broodstocks for the purposes of obtain the material to let it out in natural water bodies. Page 293 In: First Congress of Ichthyologists in Russia. Moscow, Russia. Popova, A.A., V.N. Shevchenko, L.V. Piskunova, P.V. Chernova and G.P. Marinova. 2001. Results of experimental-industrial work in creation of sturgeon fishes broodstocks. Pages 58-61 In: Problems of Present Commercial Sturgeon Breeding. Astrakhan, Russia. Samoilenko, D.A. 2011. About the factors of faster sexual maturation of sturgeon fishes. Pages 176-179 In: Sturgeon Fishes and their Future. Berdyansk, Ukraine. Vasil’eva, L.M. 2000. Biological and technological peculiarities of commercial aquaculture of sturgeon fishes in conditions of Lower Volga Region. Astrakhan, Russia. FIGURE 6. Scheme of sterlet broodstock formation. FIGURE 7. Page of a fish electronic passport that was made with a computer program.
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