WWW.WAS.ORG • WORLD AQUACULTURE • JUNE 2013 67 collected from the wild. A subculture was maintained on tryptone soy agar slopes (Himedia) at 4 C and was routinely tested for pathogenicity. A stock culture in tryptone soy broth was stored at -20 C to provide stable inoculum throughout the study (Dhanaraj and Haniffa 2011). After the 50-d feeding trial, experimental fish were injected intraperitoneally (Fig. 3) with one-day-old culture of A. hydrophila (100 µL/fish) at 106 cfu/mL. All fish were observed daily for one week to record clinical signs and mortality. Survival of striped murrel fed diets supplemented with aloe was greatest (Table 3). Survival of striped murrel fed diets supplemented with Bacillus and Indian almond was greater than that of fish fed the control diet. The improved survival suggests that production of metabolites by probiotics suppressed the pathogenicity of A. hydrophila (Fig. 5) and ultimately resulted in better survival and growth of the host. Aloe was effective as a disease suppressant and antibacterial agent in striped murrel. Substances in aloe are known to have anti-bacterial and antifungal effects (Klein and Penneys 1988, Kim et al. 1999, Sunitha 2012). Aloe is recommended as a feed supplement for treatment of EUS. Notes M.A. Haniffa, Centre for Aquaculture Research and Extension (CARE), St. Xavier’s College, Palayamkottai – 627 002, Tamil Nadu, India. Email: haniffacare@gmail.com, Website : www. caresxc.org Acknowledgments Authors acknowledge the financial assistance received from ICAR-NAIP Sub Project entitled, “A Value Chain on Murrel Production in Tamilnadu and Orissa” (F.No.1(5)/2007-NAIP dt. 22 Aug. 2008). We are grateful to Rev. Dr. Joseph S.J., Principal and Consortium Leader, St. Xavier’s College (Autonomous), Palayamkottai, for providing the necessary facilities. References Adewolu, M.A. 2008. Potentials of sweet potato (Ipomoea batatas) leaf meal as dietary ingredient for Tilapia zillii fingerlings. 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