METAGENOMICS ANALYSIS OF BACTERIAL COMMUNITY IN A TROPICAL MARINE FISH HATCHERY

Metagenomics is one of the fields to study the genetic contents of the microorganism communities directly from the environment by combination of genomic technologies and bioinformatics tool. This present a study was conducted to determine the bacterial diversity and the functional annotation in the ecosystem of tropical marine fish

The DNA was extracted from feces (combined of intestinal content and mucosa) of tiger grouper (Epinephelus fuscoguttatus) (n = 9) and Asian seabass (Lates calcarifer) ( n = 9) as well as the water samples (3 L) using commercial kit. The DNA were subjected to the 16s rRNA amplicon sequencing using the Illumina Miseq platform. The water samples (W1 and W2) showed higher richness compared to the feces samples from tiger grouper and Asian seabass (Fig. 1). Feces samples from tiger groupers (TG1 and TG2) and Asian seabass (AS1 and AS2) were dominated by order of Vibrionales, Clostridiales and Fusobacteriales meanwhile the water samples dominated by of Actinomycetales and Stramenophiles (Fig. 2). Investigations on functional annotations for all samples, using PICRUSt, revealed a number of functions related to environmental information processing, metabolism and cellular processes.