Glucose plays a key role as energy source in the majority of mammals including fish. Glucose is the most sensitive indices of the stress state of an organism. Its high concentration in blood is indicate that the fish is in stress and intensively using energy reserves i. e. glycogen in liver and muscle. An experiment was conducted to evaluate the effect of feeding and anesthetization on the glucose level of Labeo rohita fingerlings (average weight 8.0 ± 0.20 g). After feeding, blood samples were collected at different time interval such as 0, 2, 4, 8, 12, 16, 20 and 24 hr from feeding without anaesthesia and feeding with anaesthesia groups respectively. Blood glucose level was immediately analysed by using Glucometer (Dr Morepen Glucose Monitor BG-0). Glucose level reach to maximum after 8 hr of feeding and decreased towards 12 hr to the normal levels in both groups. After 24 hr of starvation the glucose level reaches to normal value. The 24 hr starvation is sufficient for any sampling procedure. Feeding have significant effect on glucose level respective to anesthetization. Clove oil is best anaesthesia did not affect significantly glucose level. Thus anaesthesia method could be used for experiment requirement glucose level.