Cronobacter sakazakii, an emerging food borne opportunistic pathogen that can survive in very dry environment. Although Cronobacter sakazakii has been a health significant in infant food products and there is a paucity of such studies in fisheries sector. The present study was aimed at determining the prevalence of Cronobacter sakazakii in fish and fishery products, molecular confirmation and characterizing the pathogen for its antibiotic resistance pattern with special reference to determining of epidemiological cut-off. 104 fresh /dry seafood samples which comprise of fish, including shellfish, mollusks, water and ice from retail markets, fishing landing centers, supermarkets of Ernakulum district, Kerala were screened. The prevalence of Cronobacter sakazakii is 8.6% and nine isolates were confirmed biochemically for Catalase & MGP (methyl-alpha-D-glucopyranoside), Oxidase reactions. Molecular confirmation was performed using PCR targeting C. sakazakii three genes (SAKA), (CRONO) and (ESS) producing amplicon size of 952bp, 199bp and 469bp respectively. Identified 9 C. sakazakii were tested for antibiotic sensitivity by disc diffusion method with an array of 20 antibiotics and determined epidemiological cut-off Antibiotic sensitivity test revealed that 66.6% of the isolates were multiple drugs resistant with resistance to carbapenams and 3rd &4th generation cephalosporins which need further confirmation either quantitatively or molecularly. The order of antibiotic resistance observed in this study were Imipenam & Cefpodoxime (88.9%) < Nitrofurantoin (77.8%) < Cefoxitin (44.4%) < (Aztreonam, Ceftazidime, Ceftriaxone, Colistin, & Moxifloxacin) at 22.2% followed by Levofloxacin & Co-trimoxazole at 11.1%. The study also revealed all the C. sakazakii was sensitive to Aminoglycosides & quinolones. The findings receives a special attention that the first report of C. sakazakii in the fish and fishery products in India and possible presence of ESBL production including carbapenamase producers.