Infection with Aphanomyces invadans is considered to be one of the serious diseases in freshwater and brackishwater fishes. Although more than 4 decades have been passed after its first occurrence in 1970's in Japan, to date, the disease causes large-scale mortalities in fishes with expanding host-range and geographical area. Importantly, there is no effective treatment available for the disease. In the current study, we have evaluated the potential of some of the drugs (ketoconazole, fluconazole & amphotericin B) and disinfectants (formalin, hydrogen peroxide and potassium permanganate) with known antifungal properties, against different life forms i.e. production and germination of spores, and growth of A. invadans under in vitro conditions. Among the drugs tested, ketoconazole was found to be comparatively more effective in arresting the sporulation, germination and growth of A. invadans compared to fluconazole & amphotericin B. Among the disinfectants tested in the present study, formalin was observed to be more effective in inhibiting production and germination of spores, and growth of A. invadans compared to hydrogen peroxide and potassium permanganate. From the results obtained, it could be inferred that ketoconazole and formalin have the potential to be used for treatment of infection with A. invadans and their combined effect needs to be evaluated.