In recent times, antimicrobial resistance (AMR) is a serious threat to global public and animal health due to development of resistance of antimicrobial drugs used in aquaculture. In this study, antimicrobial resistance in micro-organisms of brackishwater shrimp farms of Maharashtra was assessed. Sampling of P. vanamei was done from 30 brackishwater farms in Thane district of Maharashtra for a period of three months. Targeted Bacteria (E.coli, Staphylococcus spp. and Vibrio spp.) were isolated from shrimp samples, characterised by biochemical tests and test for AMR through antibiotic sensitivity test (AST) by using various antibiotics. Results of AST were interpreted by comparing the zone diameter referring to breakpoint tables based on guidelines of the Clinical and Laboratory Standards Institute M45-A2 (CLSI, 2010) and was further analysed in WHONET software.