Aquaculture America 2020

February 9 - 12, 2020

Honolulu, Hawaii

FORMULATION OF A. salmonicida ADJUVANTED VACCINE FOR RAINBOW TROUT: IMPACT OF THE ADJUVANT OIL ORIGIN

John T. Buchanan*, Melissa Allen, and Jason Stannard
Center for Aquaculture Technologies
8395 Camino Santa Fe, Suite E
San Diego, CA 92129
jbuchanan@aquatechcenter.com
 

The availability of SPF (Specific Pathogen Free) shrimp broodstock has had a significant impact on the aquaculture of shrimp; from reducing the incidence of disease to facilitating more traditional family-based breeding for shrimp. From a breeding perspective, one of the shortcomings of the SPF system is that for biosecurity reasons, it does not allow for genetics from the growing ponds to be incorporated into the breeding program. Similarly, selection for other traits such as disease resistance and carcass quality prevent broodstock on which a trait was measured from returning and contributing to the breeding nucleus. Thus, the only way to use this information for genetic improvement is to rely on family and sib-ship information. This approach is not ideal as the accuracy of selection is limited because within family genetic effects are not captured. Mass selection approaches suffer from the same short comings in biosecurity, lose potential increases in genetic gain to reduced accuracy, and suffer from the risk of inbreeding depression. Genomic selection was developed to increase the accuracy of selection, accelerate genetic gain, and consequently increase genetic gain per generation while simultaneously allowing for the control of inbreeding on a whole-genome level. It relies on the measurement of genomic similarity to predict breeding values, rather than a sib-ship relationship. This allows 1) increases in selection accuracy, 2) selection of breeding candidates from different genetic backgrounds that are more likely to perform well, 3) for the control of inbreeding (relatedness) in a genome wide fashion, 4) for selection on phenotypes that cannot be measured on the breeding candidates without depending solely on family information. This last point can have a great impact on shrimp breeding, as it would allow the accurate incorporation of genetic data from ponds without increasing biosecurity risks. Application of genomic selection is feasible when combining an economically efficient method for scanning the genome of broodstock for SNP (single nucleotide polymorphism) markers, and genomic imputation to reduce the overall burden of genotyping costs. With the development of genetic tools and understanding of breeding strategies, the economic costs for incorporating genomic selection to accelerate shrimp breeding programs are no longer barriers to implementation.