The white sea bass, Centropomus undecimalis, is a species of fishing importance with high potential for cultivation. Proper management of fish breeders requires validated anesthesia protocol that allow monitoring of reproductive maturation (cannulation), and the application of hormonal implants to induce spawning, without affecting the physiological and health status of the organisms.
The present work aims to determine the effect of sedation time on anesthesia in C. undecimalis breeders. Twenty organisms cultivated in the marine fish area of the UNAM-Sisal were used. To perform sedation (calm state), clove oil (0.003 mL per L) was used directly in the pond water. The organisms were exposed for a short (25 to 75 min) and long (100 to 125 min) sedation time, prior to exposing them to an anesthetic concentration of 0.1 mL of clove oil per L. The blood sample was obtained by puncture in the caudal vein. Plasma metabolites were evaluated using clinical diagnostic kits to determine the concentration of cortisol, glucose, lactate, hematocrit, hemoglobin concentration, and hemagglutinating activity.
A paired analysis was performed to compare the effect of sedation time. Hematological components and the time required for anesthesia (lack of response to a physical sensation), indicate similar responses (P > 0.05) in the fish of the two-sedation treatment, that is, that the sedation exposition did not generate a cumulative narcotic effect.
This protocol was probed in brood-stock of UNAM during maturation period.
Results indicate that there is no relationship between the size of the reproducers (1.2 to 6.8 kg, N=93) and the time required to reach the plane of anesthesia.
Sedation with clove oil (0.003 mL per L), prior to anesthesia, facilitated docile management of the breeders and adequate protocol for obtaining blood and gonad samples.
Acknowledgments
Financial support of National Autonomous University of Mexico (PAPIIT IN217322; IT201621)