Abstract
In several disciplines, using in vitro models means an opportunity to do more tests in less time and cost. Within these models, the screening of molecules and functions in cells is a widely used approach in biotechnology. In aquaculture, there is a challenge in improving additives and diets and understanding the mechanism of action of various compounds . Meals are an important question of diet formulation. Still . We check the effect of the different meals on the cell growth in in vitro conditions. We used generic soy meals, insect meals, animal protein and two diets for salmon, low energy and high energy. We used an artificial stomach for digestion in the pepsin meal and diet, and observed on acrylamide gels the quality of protein. Then the material is used on CHSE-214 cell line, and the growth of the culture. Our results show a different protein quality in animal meals, and the low- energy diet presents bet performance, in more fragments and lower molecular weight. The cell growth increases when using low low-energy diet or animal meals; the insect meals did not induce change, and soy meals reduce the growth of the cells. Our results suggested a complex model of action of this protein source and present evidence of the use of a protein gel and cell model for evaluating the quality of the material, for use in diet construction for fish production.
Keywords
Cells, Nutrition; Production