Sustainable aquaculture depends heavily on optimizing feed efficiency, particularly using protein sources. The high cost of fishmeal and antinutritional factors in plant-based ingredients significantly constrain productivity in intensive farming systems. The use of exogenous enzymes has emerged as a strategy to enhance nutrient digestibility and economic returns in aquaculture diets. This study aimed to evaluate the technical and economic effects of including enzyme mixtures—protease + cellulase (P/C) and xylanase + cellulase (X/C), at a 0.005% inclusion rate—in diets formulated with either fishmeal (FM) or soybean meal (SBM) for the culture of Pacific white shrimp (Litopenaeus vannamei) under commercial farming conditions. Two experimental diet groups were tested with three replicates (FM + P/C and SBM + X/C). Shrimp with an initial average weight of 1.5 ± 0.5 g were stocked at 14 shrimp per m² in 1 m3 cages ─placed in ponds from a commercial farm─ and cultured for 45 days. Productive performance variables (final weight, weight gain, survival, specific growth rate, feed conversion ratio FCR) were measured, and economic feasibility was assessed using the EBITDA indicator. Results showed significant improvements (p < 0.05) in all productive performance parameters with enzyme supplementation, including a reduction in FCR. The economic analysis revealed an increase in profitability of 34.5% with HP + P/C and 36.3% with PS + X/C diets. It is concluded that supplementation with enzyme mixtures in animal and plant diets improves the zootechnical performance and profitability of L. vannamei cultivation in commercial systems.