Aeromonas spp . constitutes a ubiquitous opportunistic pathogen with growing relevance in national aquaculture due to its capacity to acquire antimicrobial resistance and zoonotic potential . The objective was to genomically characterize Aeromonas spp. strains isolated from Amazonian aquaculture outbreaks through phylogenomic analysis , virulome and resistome . Nine strains were sequenced using Illumina NovaSeq and assembled with Unicycler v0.5.0. Species and lineages were determined by phylogenomic tree and PubMLST . Virulome and resistome were annotated with ABRicate -VFDB and AMRfinder . Zoonotic potential was evaluated with PathogenFinder2.
Three species were identified : A. caviae (n=5), A. hydrophila (n=3) and A. veronii (n=1) with multiclonal origin. The ST-1072 lineage stands out in two independent A. caviae strains in Oreochromis niloticus . All genomes presented conserved virulome including flaA , flaB , pilT , luxS , kdsA and xcpR , indicating a profile based on flagellar motility , adhesion and type II secretion . Absence of aerA and ast . Multiclass resistome detected blaOXA , tet(A) , qnrS1 , sul1 , aac(6’)-Ib-cr and cphA . All strains showed high zoonotic probability (>0.93). Amazonian strains present conserved virulome relevant for vaccines and multiclass resistome similar to Asian reports . Their high zoonotic potential requires surveillance from a One Health perspective.