Sunshine Bass are hybrids produced by crossing White Bass females and Striped Bass males. These bass hybrids are an important food fish in the United States. Hybrids show heterosis for survival, growth, disease resistance and tolerance of environmental stressors. Production time for hybrids to reach a marketable weight of 0.6 kg-1.0 kg can range between 10-20 months. During this time, females can become sexually mature and produce eggs. When stressed these females can release eggs which in turn can adversely affect water quality. It is also thought that feed energy is directed toward egg production relative to muscle growth. As with many commercially produced aquaculture species, controlling reproduction can be important to optimize growth and energy allocation efficiency in this production cycle. Triploidization is an effective method for the production of sterile fishes. This project aims to evaluate triploidy induction methods that have been used successfully with other commercial aquaculture species. Our goal is to determine an optimum treatment for triploid production with special attention being placed on maximizing embryo survival and obtaining high triploidy induction in sunshine bass. Hydrostatic pressure shocks were evaluated for the production of triploid sunshine bass embryos. Four pressures (5000, 6000, 7000, and 8000 PSI) and shock durations of 1.5, 2, 3, 4, and 5 minutes were evaluated. All shocks were administered 4 minutes post-fertilization. The produced fry were evaluated for triploidy using flow cytometry. Thermal shock was evaluated at 3 and 4 minutes post-fertilization for 10 and 15 minute cold shocks and 2 and 3 minute heat shocks (cold = 2°C and 4°C; heat = 36°C and 38°C). Initial results indicated that a high-pressure shock (8000 PSI) applied over a short period of time will produce a high percentage of triploids. A low temperature shock (2°C) applied over an extended period of time will produce a high percentage of triploids. More trials are required to fine-tune the triploidization procedure for hybrid Striped Bass.