Aquaculture America 2026

February 16 - 19, 2026

Las Vegas, Nevada

Add To Calendar 17/02/2026 14:30:0017/02/2026 14:50:00America/Los_AngelesAquaculture America 2026THE DEVELOPMENT OF TRIPLOIDY INDUCTION METHODS FOR HYBRID STRIPED BASS PRODUCTION IN COMMERCIAL AQUACULTUREChampagne 3The World Aquaculture Societyjohnc@was.orgfalseDD/MM/YYYYanrl65yqlzh3g1q0dme13067

THE DEVELOPMENT OF TRIPLOIDY INDUCTION METHODS FOR HYBRID STRIPED BASS PRODUCTION IN COMMERCIAL AQUACULTURE

Samuel García-Vázquez*, David Straus, Bonnie Brown, and Dayan A. Perera

Aquaculture/Fisheries Center

University of Arkansas at Pine Bluff

1200 University Dr. Pine Bluff, AR 71601

garcias5184@uapb.edu

 



Sunshine Bass are hybrids produced by crossing White Bass (Morone chrysops) females and Striped Bass (Morone saxatilis) males. These bass hybrids are an important food fish in the United States. Bass hybrids have a wider tolerance to environmental variables, better survival, better growth rates, and disease resistance than their counterparts. The production cycle typically ranges from 10 to 20 months to reach a marketable size of 0.6–1.0 kg. During this time, females can become sexually mature and produce eggs. When stressed these females can release eggs which in turn can adversely affect water quality. It is also thought that feed energy is directed toward egg production relative to muscle growth. As in many aquaculture species, controlling reproduction is critical to optimize growth and energy efficiency. Triploidization offers an effective method to produce sterile fish. This study evaluated induction methods for producing triploid Sunshine Bass, with emphasis on maximizing embryo survival while achieving high triploidy rates. Hydrostatic pressure shocks were tested at 5000, 6000, 7000, and 8000 PSI for durations of 1.5, 2, 3, 4, and 5 minutes, all administered 4 minutes post-fertilization. Thermal shocks were tested at 3 and 4 minutes post-fertilization: cold shocks (2°C, 4°C) for 10 and 15 minutes, and heat shocks (36°C, 38°C) for 2 and 3 minutes. The produced fry were evaluated for triploidy using flow cytometry. Results indicated that a high-pressure (8000 PSI) shock applied over a short period of time will produce a high percentage of triploids. A low temperature shock (2°C) applied over an extended period will produce a high percentage of triploids. In conclusion, triploid Sunshine Bass can be reliably produced using these treatments, though additional trials are needed to refine protocols and optimize survival for commercial applications.