Zoogoneticus tequila, more commonly known as the tequila splitfin, is a viviparous teleost fish of the family Goodeidae. Z. tequila is endemic to the Ameca River basin in Rio Teuchitlán and was thought to be extinct at two different points; (1999 and 2013). Factors such as invasive species, habitat degradation, pollution from agricultural pesticides, and factories have been an ongoing threat to Z. tequila. With the increasing levels of domestic and urban wastewater, nutrient loads, and sewage in these freshwater systems, more data on how increasing levels of anthropogenic nitrates affect the reproduction of these goodeids is needed to help aid in continuous restoration efforts in Rio Teuchitlán to be successful. For this study, Z. tequila was placed in three different concentrations in evaluating the effects of inorganic nitrates. Three tanks were a control with no additional NaNO3 added, three tanks contain 10 mg/L of NaNO3 which represent an environment of low pollution and lastly, three tanks contain 100 mg/L of NaNO3 which represent an environment with high pollution. After six months, the gestation period was longest in the 100 mg/L treatment showing that increasing concentrations of NaNO3 will likely have significant impacts on frequency of births for the species. Additionally, the number of fry produced at each birthing event was lowest in the 100 mg/L treatment. Combined, these results give an estimate of how long they will take to reproduce in the wild. These observations on how often a female gives birth will be able to answer the question of, can populations of Z. tequila remain stable after being exposed to increasing levels of nitrate pollution? Ultimately, these impacts may be useful in driving environmental remediation with the goal of restoring the population of Z. tequila.