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SCORING HISTOPATHOLOGY SYSTEM IN STUDIED SAFETY OF OXYTETRACYCLINE FOR Clarias sp

Tanjung Penataseputro, Ratna Amalia Kurniasih*, and Dwi Rahwanto
 
 Center of Fish Diseases and Environmental Investigation
 Ministry of Marine and Fisheries
Serang, PO BOX 123
 lppil_serang@yahoo.co.id

Oxytetracycline applications for overcoming problem of fish disease in catfish aquaculture have been carried out by fish farmers. It is bacteriostatic, and bactericidal in high concentration. Oxytetracycline in the body can cause gastric mucosal irritation, tissue classification, hepatotoxic (occurred in the administration of high doses) and phototoxic. The safety studied intends to find out and make sure changes in organ tissue towards catfish exposed to oxytetracycline in 2 doses with unexposed.

These safety studied was conducted using 20 fish were divided into 2 groups and maintained for 10 days. The control group is a group of fish that are not exposed to oxytetracycline. The treatment group is a group of fish that are exposed to oxytetracycline at a dose of 1 gr / 2 kg weight of fish. Histopathology identification was conducted to determine changes in organ tissue when the catfish have not been exposed nor exposed to oxytetracycline. It was done on the liver, kidney, spleen, gill, and muscle of catfish. Scoring histopathology in organ tissue is used to ascertain the significance of organ tissue changes between groups. Scoring is done by numerated the types of pathological changes like metabolic disorder, degeneration, necrosis, circulatory disorder, inflammation and tumor that occurred in catfish.

This studied showed no significant difference towards pathological changes that occur in liver, gill, and muscle (P>0,05), and showed  a significant difference in kidney (P<0,05). Necrosis and hemorrhage in kidney control group is more significant occurred compared with the treatment group. In consequence, oxytetracycline is not a factor contributing to the pathological changes for catfish in this studied.




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