THE EFFECT OF DIFFERENT EXTRACTION TECHNIQUE OF MANGROVE LEAF ON IMMUNE PARAMETER, TOTAL Vibrio SP, AND SURVIVAL RATE OF TGER SHRIMP Penaeus monodon IN LABORATORY SCALE
The use of herbs as an alternative to prevent disease in aquaculture including vibriosis. Mangrove can be use as bioremediator particularly in improving farm productivity, stabilize water quality, and can inhibit the diseases in tiger shrimp culture. It has been reported previously that the mangrove plant has high economic value and have been known to contain a bactericide. The study aims to determine the effect of extraction technique of mangrove leaf on immune parameter, total vibrio sp, and survival rate of tger shrimp P. monodon in laboratory scale.
The study was conducted in a wet laboratory of Research Institute for Coastal Aquaculture. A container of 3L-sized glass aquarium filled with sea water salinity of 28 ppt and stocked with tiger shrimp post larvae (PL 15) as many as 30 individuals each container and infected with pathogenic V. harveyi 106 CFU/mL. The treatments tested were A=Methanol extract of S. alba B=Boiling ekxtract of S. alba; C. Methanol extract S.lanceolata; D= Boiling extract of S. lanceolatan; E= Methanol extract B. gymnorrhiza; F= Boiling extract of B. gymnorrhiza, G= Positive Control (infected V. harveyi, without mangrove extract); H=Negative control (without V. harveyi and without mangrove extract). Each treatment was repeated three (3) times. Parameters observed were Vibrio harveyi population, THC, ProPo, DHC, and the survival rate of tiger shrimp.
The result showed that total Vibrio sp on the use of mangrove extract by boiling tends to be lower by about 30% compared with the methanol extract (Figure 1). Statistical analysis showed that the total hemocytes black tiger shrimp post larvae in treatment that uses methanol extract of S. alba significantly different (P <0.05) with a negative control, but not significantly different (P> 0.05) with other treatments. In the other hand PRoPo values not significantly different (P> 0.05) among all treatments (Tabel 1). The survival rate of tiger shrimp post larvae on the use boiling exctracted tehcnigue higher than the methanol extract tehcnigue (Tabel 1). The results showed that the boiling extract of mangrove likely be used for the prevention of vibriosis disease in shrimp culture.