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Add To Calendar 21/02/2017 16:00:0021/02/2017 16:20:00America/ChicagoAquaculture America 2017EFFECTS OF DIETARY PREBIOTIC AND PROBIOTIC SUPPLEMENTATION ON GROWTH PERFORMANCE AND DISEASE RESISTANCE OF HYBRID STRIPED BASS Morone chrysops X Morone saxatilis IN PONDS Salon DThe World Aquaculture Societyjohnc@was.orgfalseanrl65yqlzh3g1q0dme13067DD/MM/YYYY

EFFECTS OF DIETARY PREBIOTIC AND PROBIOTIC SUPPLEMENTATION ON GROWTH PERFORMANCE AND DISEASE RESISTANCE OF HYBRID STRIPED BASS Morone chrysops X Morone saxatilis IN PONDS

Min Ju* and Delbert M. Gatlin III
 
Department of Wildlife and Fisheries Sciences
Texas A&M University
College Station, TX 77843
jumin@tamu.edu

Prebiotics and probiotics have been shown to have a number of positive effects on various aquatic species based on laboratory studies. However, more research is needed to evaluate both additives under conditions similar to commercial production. Therefore, a feeding trial over a full production cycle was conducted with hybrid striped bass to investigate commercially available prebiotic and probiotic supplements on growth performance, immunological responses and disease resistance under conditions simulating commercial culture. Juvenile hybrid striped bass averaging 76.7 g were stocked into 12, 0.04-ha replicate ponds in May of 2015. Each of four diets supplemented with either 0 or 2% prebiotic (GroBiotic®-A, International Ingredient Corporation) and 0 or 107 CFU/g probiotic (Aquablend, BIO-CAT Microbials) in a 2x2 factorial arrangement was fed to three replicate ponds. An intermediate sampling (4 months of feeding) and a terminal sampling (11 months of feeding) were conducted to measure production characteristics, histological features of the gastrointestinal (GI) tract, intestinal microbiota and non-specific immune parameters. In addition, 40 fish per treatment were challenged by handling stress under high heat (~32 C) conditions after which mortality was recorded for 7 days.

Results to date showed: (1) no significant differences in growth performance (average final fish weight was 429.1 g); (2) the prebiotic showed a stronger effect on immunological parameters such as intracellular and extracellular superoxide production (p<0.01) (Fig. 1) compared to the probiotic; (3) fish fed the diet with prebiotic alone and both additives had significantly (p<0.001) higher survival compare to the basal group after the handling stress challenge (Fig. 2). Analysis of this experiment is ongoing and more results will be evaluated such as histology and microbiota composition of the GI tract. The goal is to have a clearer understanding of how these prebiotic and probiotic supplements perform under conditions similar to commercial production.              




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