Comparative evaluation of metabolic enzymes activities in different tissues of Pangasianodon hypophthalmus (Sauvage, 1878) fingerling reared at higher temperature

Amit Ranjan1,*, P.P.Srivastava1, K.K.Jain1 and Muralidhar P. Ande2
1 Fish Nutrition, Biochemistry and Physiology Division, ICAR-Central Institute of Fisheries Education, Off Yari Raod, Panch Marg, Versova. Mumbai - 400 061, MS, India
2 ICAR-Central Institute of Fisheries Education, Kakinada Centre, Old Burmah shell Road, Kakinada, Andhra Pradesh, India
* Corresponding Author: amitranjanfcri@gmail.com

A 60-day feeding trial was conducted to study the effect of higher temperature (32°C) in comparison to ambient temperature on different metabolic enzyme Aspartate aminotransferase (AST), Alanine aminotransferase (ALT),  Adenosine triphosphatase (ATPase), Acid Phosphatase (ACP) & Alkaline Phosphatase (ALP) activities in different tissues of Pangasianodon hypophthalmus (Sauvage, 1878). AST activity (at 37°C) differ significantly (P<0.05) in case of liver, however, temperature do not significantly affect the AST activity in case of muscle (p>0.05). The ALT activity (at 37°C) differ significantly (P<0.05) in case of liver and muscle at different temperature. ATPase activity (at 37°C) in gill do not differ significantly (P>0.05) whereas ATPase activity in liver at different temperature differ significantly (P<0.05). ACP activity (at 37°C) does not vary significantly at different temperature whereas ALP activity (at 37°C) varies significantly at different temperature in liver and intestine tissues. The present study clearly shows that there is temperature dependent change in activity of different metabolic enzyme in different tissue studied in comparison to the control at ambient temperature. The finding suggests that the metabolic responses are variable and accordingly, temperature impacts on the growth performances of this new candidate species for aquaculture.