GENE SEQUENCING AND SENSITIVITY OF BACTERIA IN Tilapia zilli FROM IJAKA-OKE LOCATION ON YEWA RIVER

A. A. Akinyemi1 , O. O. Oyelakin2, and A. R. Oloyede2
1Department of Aquaculture and Fisheries Management,
 2Biotechnology Centre, Federal University of Agriculture, Abeokuta, PMB 2240, Abeokuta. 110001, Ogun State,  Nigeria.
Corresponding author Akinyemi, A. A. adeoluakinyemi@yahoo.com
 

Bacteria were identified using the 16S rRNA gene sequencing method, and the antibiotic sensitivity test was done using five different antibiotics. Bacteria were isolated from the gut, gills and skin of Tilapia zilli from Ijaka-Oke. Bacteria DNA were extracted using CTAB method, PCR amplification was carried out using 16S universal primer for bacteria, purification of PCR product was done using ethanol precipitation, and thereafter DNAs were sequenced using automated DNA sequencer. Sequence data were analyzed on GenBank database (NCBI) using BLAST search tool to find closest related sequence. Antibiotic susceptibility patterns of isolates were also determined using Kirby-Bauer disc diffusion method. Bacteria strains were identified as Proteus mirabilis strain, Providencia vermicola strain, Providencia rettgeri strain, Morganella morganii strain, Myroides odoratimimus strain, Providencia rettgeri strain, Morganella morganii subsp. Sibonii strain, Providencia rettgeri strain, Providencia rettgeri strain, Morganella morganii strain, Morganella morganii clone, Proteus mirabilis strain, Enterobacter spp.. Cephalexin recorded highest zone of inhibition (21mm) on Enterobacter sp., followed by Erythromycin on Enterobacter spp (19mm), Lowest inhibition zone was recorded with Erythromycin on P. vermicola (10mm). P. mirabilis was susceptible to cephalexin and ceftriaxone, P. vermicola and P. rettgeri were both susceptible to erythromycin, cephalexin, streptomycin and ceftriaxone while it was resistant to cotrimoxazole. M. morgani was susceptible to streptomycin and ceftriaxone, Enterobacter sp. was susceptible to erythromycin, cephalexin, and streptomycin but resistant to ceftriaxone. Myroides odoratimimus was resistant to all antibiotics used. The study therefore showed that diverse bacterial species exist on skins, gills and guts of Tilapia zilli.