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Add To Calendar 21/02/2017 16:45:0021/02/2017 17:05:00America/ChicagoAquaculture America 2017SYNERGETIC EFFECTS OF DIETARY Bacillus subtilis WB60 AND MANNAN OLIGOSACCHARIDE (MOS) IN JUVENILE JAPANESE EEL, Anguilla japonica   Salon DThe World Aquaculture Societyjohnc@was.orgfalseanrl65yqlzh3g1q0dme13067DD/MM/YYYY

SYNERGETIC EFFECTS OF DIETARY Bacillus subtilis WB60 AND MANNAN OLIGOSACCHARIDE (MOS) IN JUVENILE JAPANESE EEL, Anguilla japonica  

Dept. of Marine Bio-materials and Aquaculture/
Feeds and Foods Nutrition Research Center (FFNRC)
Pukyong Nat'l University, Busan 608-737, Rep. of Korea
 
Jin-Hyeok Lee*, Seunghan Lee, Sungchul C. Bai

The current experiment was conducted to evaluate the synergetic effects of dietary Bacillus subtilis WB60 and mannan oligosaccharide (MOS) in juvenile Japanese eel, Anguilla japonica. Seven diets were formulated to contain three different B. subtilis levels (0, 0.5×107 and 107 CFU/g kg-1 diet, BS0, BS0.5 and BS1) with two different MOS levels (0 and 5g kg-1 diet, M0 and M0.5) and oxytetracycline at 5 g kg-1 diet (OTC). Seven diets, in a 3×2 factorial design (BS0M0(CON), BS0M0.5, BS0.5M0, BS0.5M0.5, BS1M0, BS1M0.5) and OTC, were fed to triplicate groups of fish averaging 9.00±0.11g (mean±SD) for 8 weeks. Fish were fed one of the experimental diets at 1.5 ~ 2.0% of wet body weight per day. At the end of 8 weeks of feeding trial, weight gain (WG), feed efficiency (FE), specific growth rate (SGR) and protein efficiency ratio (PER) of fish fed BS0.5M0.5 and BS1M0.5 diet were significantly higher than those of fish fed CON, BS0.5M0 and OTC diet. Whereas, on WG, FE, SGR and PER, there were no significant difference among the fish fed BS1M0, BS0.5M0.5 and BS1M0.5 diet. Nonspecific enzymatic activities including lysozyme and myeloperoxidase (MPO) from fish fed BS0.5M0.5, BS1M0.5 and OTC diet were significantly higher than those from fish fed CON, BS0.5M0, BS0M0.5 diet (P < 0.05). Parameters for intestinal morphology and histology suggested a healthier gut for the fish fed BS0.5M0.5 and BS1M0.5 diet as compared to fish fed CON and OTC diet. Whereas, results from the disease challenge test with bacteria Vibrio angulillarum showed significantly lower survival rate for fish fed CON diet than those of fish fed other experimental diets. Therefore, these results indicated that B. subtilis at 0.5×107CFU/g kg-1 diet with mannan oligosaccharide (MOS) could have synergetic effects on the growth performance, immune responses and intestinal condition in Japanese eel.

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