World Aquaculture Society Meetings

Add To Calendar 25/02/2016 16:30:0025/02/2016 16:50:00America/ChicagoAquaculture 2016CuZnSOD, AN ANTIOXIDANT GENE FROM SEAHORSE Hippocampus abdominalis; CLONING, SEQUENCE CHARACTERIZATION, IMMUNE RESPONSE AND ANTIOXIDANT ACTIVITY OF ITS RECOMBINANT PROTEIN   Concorde AThe World Aquaculture Societyjohnc@was.orgfalseanrl65yqlzh3g1q0dme13067DD/MM/YYYY

CuZnSOD, AN ANTIOXIDANT GENE FROM SEAHORSE Hippocampus abdominalis; CLONING, SEQUENCE CHARACTERIZATION, IMMUNE RESPONSE AND ANTIOXIDANT ACTIVITY OF ITS RECOMBINANT PROTEIN  

N. C. N. Perera1,2*, G. I. Godahewa and Jehee Lee
E-mail: imarshana@gmail.com
 
1Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University, Jeju Self-Governing Province 690-756, Republic of Korea
2Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province 690-756, Republic of Korea

Once the life on earth evolved in the presence of oxygen, a large battery of antioxidant systems also necessarily adapted by evolution. CuZnSODs are a group of SOD which catalyzes the dismutation of superoxide into oxygen and hydrogen peroxide. The structural role facilitates by the neighboring Zn+2 and while disproportionate the superoxide anions via its Cu+2 ion redox cycle. In silico characterization was conducted using bioinformatics tools and webservers. Spectrophotometric method was used to investigate the antioxidant activity of the rHaCuZnSOD through the conventional xanthine/xanthine oxidase (XO) assay. Flow cytometer analysis was performed in order to investigate the H2O2 scavenging activity of the recombinant protein with the presence of HCO3-. Moreover, MTT assay was conducted to find the cell viability % of rHaCuZnSOD.

The putative HaCuZnSOD cDNA was comprised of 842 bp encodes for a peptide of 154 aa with a predicted molecular mass of 15.94 kDa and a theoretical pI value of 5.73. The low instability index, 14.98 predicted the HaCuZnSOD protein to be stable. Optimum results of the xanthine/XOD assay were gained under the pH 9 (Fig.1. X) and at 25 °C temperature (Fig.1. Y). Highest SOD activity was showed by the highest protein concentration, 160 µg (Fig.1. Z). The highest reduction of the intracellular ROS was detected in 100 µg/mL of rHaCuZnSOD where the highest cell viability % was given by the same protein concentration. Moreover, the highest expressional level of HaCuZnSOD was found to be in the blood tissue. The temporal assessment under the pathological stresses showed a late response by the HaCuZnSOD. Overall findings demonstrated the biological functions of HaCuZnSOD and it may identify as an antioxidant which could play a crucial role in seahorse against the oxidative stress.




Copyright © 2001-2019 World Aquaculture Society All Rights Reserved.