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EFFECT OF CO2 CONCENTRATION ON THE TRANSCRIPTOME PROFILE IN MICROALGA Chlorella pyrenoidosa  

Xue Sun, Jia Shen, Nian-Jun Xu∗
 
School of Marine Sciences, Ningbo University
Key Laboratory of Marine Biotechnology of Zhejiang Province
Zhejiang Ningbo 315211, P. R. China
Corresponding author's E-mail: xunianjun@nbu.edu.cn

In order to enrich our knowledge of CO2 concentrating mechanisms (CCM) in the eukaryotic algae, we used high-throughput sequencing technology to acquire transcriptome profiles at different CO2 levels in unicellular green alga Chlorella pyrenoidosa. Altogether 53.86M and 62.10M clean short reads of 100 nt were obtained by Illumina sequencing from algae cultured at 4-fold air CO2 and air CO2 concentrations, respectively. A total of 32,662 unigenes were assembled from the two pooled samples. With an E-value cut-off of 1e-5, 7348, 5954 and 9092 unigenes were annotated in Gene Ontology (GO), Eukaryotic Orthologous Groups of proteins (KOG) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases, respectively. After screening, 51 differentially expressed unigenes were up-regulated and 8 were down-regulated in air CO2 group, 4-fold air CO2 group as the control. Eight differentially-expressed unigenes were validated their transcript levels by real-time quantitative PCR. Of them, thioredoxin-like protein, Laminin subunit beta-1 and Chlorophyll a/b binding protein might be associated with the utilizing of inorganic carbon at air CO2 level.

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