World Aquaculture Society Meetings

Add To Calendar 23/02/2016 15:30:0023/02/2016 15:50:00America/ChicagoAquaculture 2016RECENT STUDIES ON ACUTE HEPATOPANCREATIC NECROSIS DISEASE (AHPND, ALSO KNOWN AS EARLY MORTALITY SYNDROME, EMS)   Champagne 1The World Aquaculture Societyjohnc@was.orgfalseanrl65yqlzh3g1q0dme13067DD/MM/YYYY

RECENT STUDIES ON ACUTE HEPATOPANCREATIC NECROSIS DISEASE (AHPND, ALSO KNOWN AS EARLY MORTALITY SYNDROME, EMS)  

Jee Eun Han*, Kathy FJ Tang, Donald V. Lightner
 
School of Animal and comparative biomedical sciences, University of Arizona, Tucson, Arizona  85721
Email: jeehan@email.arizona.edu

AHPND diagnosis (PCR & qPCR)

Initially, diagnosis of Acute hepatopancreatic necrosis disease (AHPND) could only be accomplished through histological examinations and laboratory bioassays, but recently, several molecular diagnostic methods based on conventional PCR those targeting pirAvp and pirBvp genes became available. In addition, for monitoring disease progression, a qPCR method targeting the pirAvp gene was developed to detect and quantify the virulence plasmid in the AHPND affected shrimp.

AHPND transmission (Typing PCR)

We found geographic sequence variations in the virulence plasmid between AHPND America and Asia isolates (Table) and the results suggest that the Asia isolates were different from the Mexico (Central America) isolates (Figure). This method (genetic variation) can be used as markers for monitoring the spread of this disease.




Copyright © 2001-2019 World Aquaculture Society All Rights Reserved.