IMMOBILIZATION OF BACTERIAL BIOCONTROL AGAINST PATHOGENIC BACTERIA IN FISH USING VARIOUS MATRICES
Probiotics including biocontrol are commanly used in closed or semi-closed aquaculture. Application of probiotics in open waters aquaculture, for example in cage aquaculture, may less effective because of the high possibility of spread out of probiotics in open ocean. Therefore, apropriate matric for immobilization of probiotics is needed for open waters aquaculture. The purposes of this research was to find out an appropriate matric for immobilization of bacterial biocontrol against fish pathogenic bacteria. Several matrices i.e. polyester, microfiber, alginat, caregeenan and agar were used to immobilize bacterial biocontrol, strains RLP1, MIR2 and S2V2. Different immobilitation techniques were applied in this study depond on the matrice used. The evaluation was conducted by enumerating viable bacteria on each matric after 24 h post immobilization process.
Results indicated that viable count of each strain varied depend on nature of the matrices. RLP1 strain exhibited high bacterial viability on microfiber, κ-carageenan and agar. Whereas high bacterial viabilities of MIR2 strain were found on the matrices of microfiber, Ca-alginat and agar. High bacterial viabilities of S2V2 strain were found on the matrices of microfiber, agar and κ-crageenan. The three bacterial strains exhibited high bacterial viability on the immobilization matric of microfiber. Ketiga strain bakteri juga diuji untuk immobilisasi pada matrik microfiber. On this matric, bactrial viable count ranged 1,5 x 107 - 1,17 x 1011 CFU/cm2. The highest bacterial viable count was exhibited by strain RLP1 strain (3,18 x 1010 CFU/cm2), followed by S2V2 strain (2,00 x 1010 CFU/cm2) and MIR2 strain (1,74 x 1010) (Tabel 1). Microfiber might be developed as immobilization matric of bacterial biocontrol in aquaculture as microfiber is cheap, available commercially, and applicable by using a simple immobilization process. This study find out cheap and appropriate matric for immobilization of bacterial biocontrol to counter measure the bacterial fish pathogen.