STUDY  ON MILKY DISEASES  INFECTION IN WILD AND CULTURED LOBSTER, Panulirus homarus  IN INDONESIA

*Ketut Sugama1, N.L Anggra Lasmika2, Isti Koesharyani1 and Clive Jones3
1,. Center for Research and Development of Aquaculture,  Jakarta Indonesia
2   Lombok Quarantine office, West Nusa Tenggara, Indonesia
3  Centre for Sustainable Tropical Fisheries and Aquaculture, Collage of  
 Marine environmental Sciesnces James Cook University Australia
 ketut_sugama@yahoo.com

Milky Haemolymph Disease (MHD-SL) in spiny lobster (Panulirus homarus)  is the most pathogenic diseases.  Research on MHD-SL infection has not been undertaken in Indonesia. Therefore present study tries to determine pathogenic level and treatment to overcome of MDH-SL. The MHD-SL was confirmed by using DNA molecular technique. The study also tries to find an option for MHD-SL treatment. The spiny lobster samples were collected from Awang Bay, Telong-Elong Bay and Gerupuk Bay of Lombok Island and samples were analyzed in laboratories. MHD-SL was confirmed by PCR with specific primer of 254 bp and histology by staining HE and gram twort to finds tissue damaged and Rickettsia Like Bacteria (RLB). OTC test was determined by LC50.

The result showed that infected MHD-SL was found only in cultured spiny lobster on cage located  at  Gerupuk Bay. Determination of LC50 was found at diluted of MHD-SL10-3,2. and infected MHD-SL could be treated by Oxytetracycline (OTC) at a dosage of 0.01 ml/100 g of weight, and after 3 weeks of treated by OTC  shows that all 35 lobster treated were all survived and negative MHD-SL. Picture below shows  the spiny lobster not infected and infected MHD-SL