Effect of AQUAVI® Met-Met supplementation on the success of reducing fishmeal levels in diets for shrimp reared in green water conditions  

Orapint Jintasataporn1*, Cláudia Figueiredo-Silva2, Dhanapong Sangsue3
1Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Bangkok, Thailand
2Evonik Industries, Nutrition and Care, Animal Nutrition, Hanau, Germany
3Evonik (SEA) Nutrition and Care, Animal Nutrition, Singapore
*Email: ffisora@ku.ac.th
 

The objective of this study is to evaluate efficacy of 200% for AQUAVI®Met-Met, 100% for DL-Methionine and 65% for MHA-Ca in meeting the methionine requirement of white leg shrimp (Litopenaeus vannamei) fed with low fishmeal diets in green water conditions.

The study was assigned in a completely randomized block design, with 8 treatments and 6 replications in 2 ponds with each of 3 replications. The treatments were fishmeal20%(T1FM20), the other 3 treatments of 10%fishmeal with different forms and levels of methionine source: DL-Methionine 0.22%(T2FM10-DLMet0.22), AQUAVI®Met-Met 0.11% (T3FM10-Met-Met0.11), MHA-Ca 0.34% (T4 FM10-MCa0.34) and other 4 treatments of 5%fishmeal: DL-Methionine 0.26%(T5FM5-DLMet0.26), AQUAVI®Met-Met 0.13%(T6FM5-Met-Met0.13), AQUAVI®Met-Met 0.23% (T7FM5-Met-Met0.23) and MHA-Ca 0.40% (T8 FM5-MCa0.40). Shrimp were reared in 2*2.5*0.9 m net cage at stocking density of 100 pieces/m2 in brackish water of 10 ppt. Shrimps of 0.57±0.02g were allocated to treatment cage in each pond. The growth parameters measured were production (g/cage) body weight (BW, g/ind.), body weight gain (BWG, g/ind.), average daily gain (ADG, g/ind./d), specific growth rate (SGR, %/d), feed intake (FI, g/ind.), daily feed intake (DFI, g/ind./d), feed conversion ratio (FCR), survival rate (SR, %), after 10 weeks of study.

The results after 10 week study showed that supplementation different levels of methionine in forms of DL-Methionine, AQUAVI®Met-Met,  and MHA-Ca (T2-T8) showed the same (p>0.05) growth performance, feed utilization and survival rate as shrimp fed fishmeal 20%(T1).

The proximate composition of shrimp (whole body) showed significantly different (p<0.05) between forms and levels of methionine on moisture, protein and energy. The lipid content in shrimp body showed no significantly different (p>0.05). Over all of nutrient deposition in group of shrimp fed high levels of DL-Methionine, AQUAVI®Met-Met, and MHA-Ca in 5%fishmeal diet exhibit the high protein, lipid and energy accumulation.

Therefore, supplemental DL-Methionine, AQUAVI® Met-Met, and MHA-Ca in fishmeal reduction diets (5-10%) for shrimp reared in green water conditions can improve shrimp production, feed utilization and survival rate closed to diet of 20% fishmeal.