EFFECTIVENESS OF POWDER PROBIOTIC CULTURED IN SEAWATER WITH MOLASSES AGAINST VIBRIO IN WHITE SHRIMP Litopenaeus vannamei

In the case of bacterial desease, vibriosis was the chief problem particularly in the stages of the shrimp's life history. Vibrio infection can lead to necrosis, melanisation, even mass mortality of shrimp. An alternative method to prevent it by using probiotic. Probiotic can be found in powder form because powder probiotic can make it long term to keep and stability in quality. However, some cases indicated that vibrio in ponds was still high althought the powder probiotic has been applied. The objective of this study was to evaluate the effectiveness of powder probiotic cultured in seawater with molasses against vibrio.

White shrimp (Litopenaeus vannamei) was captive in four of 60.000 m³ ponds. Total of 0,1 ppm powder probiotic cultured in seawater with 0,2 ppm molasses as carbon source. The data analized was probiotic colony growth in probiotic culture, vibrio colony growth in ponds, and water quality of ponds for one month. The result of probiotic culture showed instead of probiotic colony, dominant colony growth was vibrio with 93% Vibrio parahaemolyticus. The seawater wasn't steril so it still contain vibrio, and probiotic was defeated. The result of vibrio colony growth in ponds showed probiotic can controlled green vibrio but still can't defeated yellow vibrio. Green vibrio can be pressed until 1x10¹ cfu/mL to 1x10² cfu/mL while yellow vibrio stood in 1x10³ cfu/mL to 1x10⁴ cfu/mL. The result of water quality of ponds was ammonia can be reduced between 0,06 ppm to 0,56 ppm but nitrite was still high. It means that activity of probiotic was not finished to nitrate.

Based on this study, it can be concluded that powder probiotic cultured in seawater with molasses still ineffective contribution to defeated against vibrio in white shrimp (L. vanname), in case of seawater that used to culture was directly taken from the sea. Seawater with sterilized treatment would be recommended before applied in probiotic culture to clear it from vibrio.

Keywords: Powder probiotic, Vibrio, bacterial desease, white shrimp