EVALUATION OF CRYOPROTECTANT (Methanol, Glycerol and Dimethylsulphoxide) FOR SPERM CRYOPRESERVATION OF TIGER SHRIMP Penaeus monodon

Rosmiati and  Ike Trismawanti
Research Institute for Coastal Aquaculture
emirosmiati@yahoo.com .

The lack of wild male broodstock shrimp tiger as the spermatophore source, unconcurrent of gonad maturation period of female and male parent and unsynchronism between spermatophore (donor) supplying and female parent molt cycle as the receiver is required to do spermatophore supplying throught cryopreservation technique development. One of The spermatophore cryopreservation successfully is determined by cryoprotectant used. The study aims to evaluate the best cryoprotectant used to sperm cryopreservation of tiger shrimp. Sperm cryopreservation was carried out by using extender of saline solution and cryoprotectant of methanol, glycerol and dimetylsulfokside at the concentration of 5 % in sterile saline solution and keep for 30 days at the room temperature, -20oC and liquid Nitrogen (-196oC).  The observation of sperm mortality was investigated at the initial and every 5 days of rearing time period. Findings showed that among the three of cryprotectant used, glycerol was obtained to be the best cryoprotectant for sperm cryopreservation of tiger shrimp with the density of sperm and survival rate percentage of sperm were 0.8 x 106 cell/mL and 80.4 % at -196oC (liquid nitrogen) after 30 days of rearing period.