GROWTH PERFORMANCE OF TRANSGENIC TIGER SHRIMP BROODSTOCK (Penaeus monodon) AT DIFFERENT GENERATION
The provision of tiger shrimp (Penaeus monodon) broodstocks is part of the overall sustainability of tiger shrimp under hatchery production. Diseases resistant genes assemblage for tiger shrimp has been initiated by The Research Institute for Coastal Aquaculture (RICA), Maros, through transgenesis approach under anti-virus genes transfection. Gene antivirus was successfully transferred to the embryo from wild type broodstock to producing tiger shrimp founder (F0). The breeding of founder (F0) transgenic tiger shrimp has been conducted to produce F1 generation. The present study aimed to evaluate the growth performance of broodstock candidates of tiger shrimp at different generation (F0 and F1).
This research was conducted at 4 ponds with size of 2000 m2 in the experimental shrimp pond installation in Takalar Regency, South Sulawesi.. The broodstocks of differences generations as the treatment, which were: broodstocks originated from F0 generation, (A) and F1 (B). The 22.63 to 28.57 g of broodstock candidates were stocked 0.5 ind.m-2 and then reared for 128 days. During rearing period, these shrimp were fed using commercial pelleted feed with content 36-38% of protein in dosage of 10-4%/ body weight. Feeding frequency was applied for two times per day in the morning and in the evening. Measured variables were growth in weight, daily growth rate, size distribution, survival rate of tiger shrimp broodstock and Water quality, i.e temperature, salinity, dissolved oxygen, pH, alkalinity, nitrate, nitrite phosphate and TOM were monitored every week.
The results of this study indicated that the performances of these shrimps, growth rates, size distributions as well as survival rates between these F0 and F1 were not significantly different (p>0.05). Trend the growth pattern of tiger shrimp during the 128 days of rearing period on the each treatment relatively equal (Figure 1). Water quality measured during the experiment was still within the range of acceptable values for the growth of tiger shrimp.