A GENETIC DIVERSITY STUDY OF MARBLE GOBY (Oxyeleotris marmorata) IN MALAYSIA AND TAIWAN USING MITOCHONDRIAL D-LOOP AS MARKER

Hao-Hsuan Hsu, Chaiw-Yee Teoh and Tzong-Yueh Chen*
Department of Biotechnology and Bioindustry Sciences, Institute of Biotechnology, Translational Center for Marine Biotechnology, and Agriculture Biotechnology Research Center, National Cheng Kung University, Tainan 70101, Taiwan
Department of Agricultural and Food Science, Faculty of Science, Universiti Tunku Abdul Rahman, Jalan Universiti, Bandar Barat, 31900, Kampar, Perak, Malaysia
Email: zhuangtz91@gmail.com

In this study, genetic diversity of marble goby in Malaysia was analyzed using published mitochondrial D-loop region, wild type and farmed-breed marble goby from Malaysia was compared with farmed-breed ones from Taiwan to investigate the genotype differences between wild type and farmed-breed marble goby from Malaysia and to determine the genetic differences of marble goby species from Malaysia and Taiwan. The result shows that the haplotype of farmed-breed marble goby from Malaysia with the wild type contain significant differences, and the haplotype was found to be close to the haplotype of farmed-breed marble goby from Taiwan, indicating breed from this two region could inherit from same relative's species.

Malaysia wild type marble goby samples were collected from Tapah, Air Kuning (sample WT1, WT2) and Malim Nawar, Kinta River (WMM1, WMM2) while farmed-breed ones was collected from Johor Bahru, Kampung Teluk Jawa (FJB1, FJB2). While, Taiwan farmed-breed ones were collected from Guanmiao, Tainan, Taiwan (FTW1, FTW2). Total 8 samples were analyzed with D-loop region in mitochondria analysis, in the full length 817 bp nucleotides, 24 SNP sites were found in the samples and classified into different haplotypes. From genetic distance matrix analysis, we found there is significant difference between the farmed-breed and wild type haplotypes from Malaysia. However, farmed-breed haplotypes in Malaysia and Taiwan shows low genetic distance. Besides, the haplotypes of wild type contain certain genetic variation within their group. From the result of SNP sites analysis, there are 5 SNP sites found to be the specific diversify sites between wild type and farmed-breed, indicating the evolution of marble goby from different coastal region in same population.