CROSS BREEDING OF SUSTAINABLE HYBRID, HYPOPHTHALMICTHYS MOLITRIX () X ARISTRICTHYS NOBILIS (), WITH INTRAMUSCULAR INJECTION OF OVAPRIM-C AT ISLAMABAD, PAKISTAN  

Muhammad Naeem, Amina Zubari,Abdus Salam and Saima Yousaf
Institute of Pure and Applied Biology, Bahauddin Zakariya University, Multan, 60800, Pakistan
(phone: +92-61-9210053; fax: +92-61-9210068; e-mail dr_naeembzu@yahoo.com

The experiment was planned to observe production of hybrid from sustainable hybrid fish, Hypophthalmicthys molitrix (♀) x Aristricthys nobilis (♂), by using single intramuscular injection of Ovaprim-C, number of eggs Kg-1, fertilization rate and hatching percentage was studied during May - June, 1996 in fish hatchery Islamabad Fishes, Pakistan (Table-IV) were spawned and  hybrid produce  successfully following a single dose of Intramuscular injection of Ovaprim (LH-RH analogue) with 0.6 ml Kg-1 for female(Table-II)  and 0.1 ml Kg-1 for male ova and milt were stripped simultaneous and mixture was stirred for 15 - 30 seconds during which fertilization occurred. Hatching occurred within 18 - 32 hours after fertilization. In the present study, ovulation, fertilization and hatchling rate values were estimated to 100, 81.62 and 74.77%, respectively. (Table-I)  Regression analysis was applied to assess body weight influence on absolute (Table-III)