EFFECT OF PROBIOTIC ON OSMOLALITY AND SOME SEMINAL PLASMA IONS OF RAINBOW TROUT Oncorhynchus mykiss BROODSTOCK

 Erfan Akbari Nargesi, Bahram Falahatkar*
 
 Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Guilan, Iran
 E-mail: falahatkar@guilan.ac.ir
 

Seminal plasma produced by the sperm duct provides an ionic environment that maintains the viability of spermatozoa after their release from the testis. As a result, some parameters such as seminal plasma ions and osmolality can affect sperm quality. Also, according to previous studies, proper nutrition can improve sperm quality. This study aimed to investigate the effect of probiotic on osmolality and some seminal plasma ions of rainbow trout broodstock. For this purpose, 44 males of rainbow trout broodstock with an average initial weight of 1365.7 ± 33.40 g (mean ± S.E.M) were fed for 12 weeks with diets containing 0, 0.5, 1 and 2 g/kg Bio-Aqua probiotic. During the feeding period, fish divided into four groups (n = 3-4 in each replicate) in a raceway ponds. For each treatment, 3 replicates were considered. At the end of the experiment, seminal plasma was collected after semen centrifugation of three adult males from each replicate. The results of the present research are shown in Table 1. According to the results, the highest level of Ca2+ was related to 1 g/kg treatment and was significantly different with 0.5 g/kg and control treatments (P<0.05). The highest level of Mg2+ was observed in 2 g/kg treatment and significantly different with control treatment (P<0.05). Furthermore, the highest level of Na+ was observed in 1 g/kg treatment and significantly different with control treatment (P<0.05). K+ level was not significantly different among treatments (P>0.05). As well as, a significant difference was observed in osmolality between 0.5 g/kg treatment and control treatment (P<0.05). The results of the present study demonstrated the ability of probiotic to influence on osmolality and seminal plasma ions in rainbow trout broodstock. In this regard, results showed that use of probiotic in rainbow trout broodstock diet can improve sperm quality.