WWW.WAS.ORG • WORLD AQUACULTURE • SEPTEMBER 2019 69 Conclusions Although red tilapia farmed in seawater (35 g/L) at two densities showed values of packed cell volume, mean corpuscular volume, total protein, albumin and cholesterol within the range of those farmed in fresh water at low and high densities (Hrubec et al. 2000), our study found erythrocyte count and glucose were lower, while hemoglobin concentration, and mean corpuscular hemoglobin concentration were higher than that study. This suggests the adaptation of red tilapia to the increase in water salinity also affects its hematological response. This information must be taken into consideration for its use as diagnostic tools when compare within the same species. The hematological and blood chemistry of juvenile red tilapia suggest that the addition of probiotics to marine systems with biofloc promotes better nutrition and health status of the fish, even at high densities. On the other hand, more information about the blood of red tilapia cultured in marine water is needed to determine normal physiological values under different culture conditions. Acknowledgments We appreciate the funding provided by the project CONACYT CB 2015-1/253151 and scholarship # 28240. We thanks to the students Jesus, Ángel, Juan Jose, Ricardo, Roberto and Brianda for their hard work and participation during the experiment. Notes Bañuelos-Vargas*, O.A. Mendoza-Gamboa, G.A. Rodríguez-Montes de Oca, E., J.C. Román-Reyes, Facultad de Ciencias del Mar, Universidad Autónoma de Sinaloa, Mazatlán, Sinaloa, México. Email: garm73@gmail.com * Corresponding author: Isaura Bañuelos-Vargas. Facultad de Ciencias del Mar. Paseo Claussen s/n. C.P. 82000. Tel: 669 828656. Email: dra.mariabanuelos@uas.edu.mx Martínez-Montaño, Universidad Politécnica de Sinaloa (UPSIN), Mazatlán, Sinaloa, México. Email: emartinez@upsin.edu.mx; Dirección de Cátedras-CONACYT, CONACYT, Ciudad de México, México. Email: emartinez@upsin.edu.mx References Abdul A., C.K. Kartik and S, Subrata. 2012. Effect of salinity on the oxygen consumption of tilapia fingerlings. DIU Journal of Science and Technology 1(7):12-14. Abduljabbar A.A., A.M. Nour, T. Srour, N. El-bermawy, W.A. Fayed and A.T. Mansour. 2015. Intensive Nile Tilapia (Oreochromis niloticus) production under biofloc technology systems. Global Journal of Fisheries and Aquaculture (GJFA) 2: 64- 80. Atencio-García, V., F. Genes-López, D. Madariaga-Mendoza and S. Pardo-Carrasco. 2007. Hematología y química sanguínea de juveniles de Rubio Salminus affinis (Pisces: Characidae) del río Sinú. Acta Biológica Colombiana12S:27-40. Bahmani M., R. Kazemi and P. Donskaya. 2001. A comparative study of some haematological features in young reared sturgeons (Acipenser persicus and Huso huso). Fish Physiology and Biochemistry 24:135-140. Blaxhall P.C. and K.W. Daisley. 1973. Routine haematological methods for use with fish blood. Journal of Fish Biology 5(6):771781. Clauss T.M., A.D. Dove and J.E. Arnold. 2008. Hematologic disorders of fish. Veterinary Clinical of North America: Exotic Animal Practice 11(3):445–462. D’Abramo L.R. 2018. Fulfilling the potential of probiotics, prebiotics, and enzymes as feed additives for aquaculture. The Journal of the World Aquaculture Society 49(3):444-446. De Verdal H., W. Rosario, M. Vandeputte, N. Muyalde, P. Morissens, J-F. Baroiller and B. Chevassus. 2014. Response to selection for growth in an interspecific hybrid between Oreochromis mossambicus and O. niloticus in two distinct environments. Aquaculture 430:159-165. Grant, K.R. 2015. Fish Hematology and associated dissorders. Veterinary Clinical of North America: Exotic Animal Practice 18:681-701. Hasan A., A. Hilali and M.S. Al-Khshali. 2016. Effect of water salinity on some blood parameters of common carp (Cyprinus carpio). International Journal of Advanced and Applied Sciences (IJAAS) 2(1):17-20. Hrubec, T.C., J.L. Cardinale and S.A. Smith. 2000. Hematology and plasma chemistry reference intervals for cultured tilapia (Oreochromis hybrid). Veterinary Clinical Pathology 29:7-12. Hrubec, T.C. and S.A. Smith. 2010. Hematology of fishes. Pages 9941003 In: D.J. Weiss and K.J. Wardrop, editors. Schalm’s Veterinary Hematology. Wiley-Blackwell, NY, NY USA. Ma, F., X.Q. Li, B.A. Li and X.J. Leng. 2015. Effects of extruded and pelleted diets with differing protein levels on growth and nutrient retention of tilapia (Oreochromis niloticus × O. aureus). Aquaculture International 23:1341-1356. Maciak, S., K. Janko, L. Kotusz, L. Choleva, A. Boron, D. Juchno, R. Kujama, J. Kozlowski and M. Konarzewski. 2011. Standard metabolic rate (SMR) is inversely related to erythrocyte and genome size in allopolyploid fish of the Cobitis taenia hybrid complex. Functional Ecology 25:1072-1078. Martínez-Porchas M., L.R. Martínez-Córdova and R. RamosEnriquez. 2009. Cortisol and glucose: Reliable indicators of fish stress? Pan-American Journal of Aquatic Sciences (PANAMJAS) 4(2):158-178. Mazzaferro, E.M., E. Rudloff and R. Kirby. 2002. The role of albumin replacement in the critically ill veterinary patient. Journal of Veterinary Emergency and Critical Care (JVECC) 12:113-124. Miranda-Baeza A., M.A. Marizcal-López, J.A. López-Elías, M. Rivas-Vega, M. Emerenciano, A. Sanchez-Romero and J.L. Esquer-Méndez. 2017. Effect of inoculation of the cyanobacteria Oscillatoria sp. on tilapia biofloc culture. Aquaculture Research 48(9):4725-4734. Telli, G., M.J. Ranzani-Paiva, T.D. Carla-Dias, F. Rosa-Sussel, I. Massa-toshi and L. Tachibana. 2014. Dietary administration of Bacillus subtilis on hematology and non-specific immunity of Nile tilapia Oreochromis niloticus raised at different stocking densities. Fish and Shellfish Immunology 39(2):305-311. Valenzuela, A., K. Alveal and E. Tarifeño. 2002. Haematological response of the trout (Oncorhynchus mykiss Walbaum 1792) to the acute hypoxic stress: red blood cells. Gayana 66(2):255-261. Yousefi M., M. Paktinat, N. Mahmoudi, A. Pérez-Jiménez and S.M. Hoseini. 2016. Serum biochemical and non-specific immune responses of rainbow trout (Oncorhynchus mykiss) to dietary nucleotide and chronic stress. Fish Physiology and Biochemistry 78:140-157.
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