This study evaluated the effect of brown seaweed coating on the quality of reef cod(E.diacanthus) fillets stored under refrigeration. Treatments: T1(control) air packaging, T2(vacuum packaging), T3(air packaging+2%brownseaweed coating) and T4(vacuum+2% brown seaweed coating). TVB-N, TMA-N, PV, TBA value showing sign of deterioration increased during refrigerated storage. Biochemical values were relatively higher in reef cod fillets stored in air pack followed by vacuum packing. This coating showed the higher antioxidant and preservative effects with vacuum packed. E.coil and S.aureus was not detected in any of the samples. Among all treatments, T4 was the most effective one to inhibit the biochemical microbial and sensory spoilage process. Overall, brown seaweed extract coating(2%,w/v) and vacuum packaging showed a synergistic effect for shelf life extension. Brown seaweed extract has the potential to explore as a natural antioxidant and antimicrobial agent for preserving of reef cod fillet during refrigerated storage for extended shelf life.