SPERM QUALITY OF JUNDIÁ Rhamdia quelen AFTER ACTIVATION BY DIFFERENT SOLUTIONS

The Rhamdia quelen is a native catfish of good commercial acceptance and adaptation at management in Latin American. Therefore, studies that improve the reproduction technique are necessary, main the related to sperm quality.

The aim was to evaluate the sperm quality of Rhamdia quelen using different activating solutions. The factorial experimental design was used. The factors were: 1) Activating solutions contained, fructose, glucose, sucrose, NaHCO₃ and NaCl; 2) Solutions molarity of 40, 80, 120mM. The distilled water (0mM) as considered as control. For so, nine males were selected for reproduction performing three semen pools (three males each) that were subjected at activating solutions in triplicate (experimental units). After activation by different solutions, the computerized parameters of percentage of sperm motility and velocities were evaluated using the IMAGEJ software/CASA plugin (Computer Assisted Sperm Analysis). There was no factorial effect (P>0.05), however, there was an main component effect (P<0.01) of the solutions only for the sperm motility rate (MOT) (Figure 1) with lower values for NaHCO₃ and NaCl solutions, and effect (P<0.01) of molarity to velocities (VCL, VSL) (Figure 2) with better results for solutions containing 120mM. It was concluded that activating solutions containing glucose, sucrose and fructose in the concentration of 120 mM are indicated for the sperm activation of Rhamdia quelen, promoting better spermatic qualities.