Aquaculture Africa 2021

March 25 - 28, 2022

Alexandria, Egypt

DNA BARCODING AND MORPHOLOGICAL IDENTIFICATION OF EGYPTIAN MARINE SHRIMP SPECIES

Eman M. Abbas, Hala M. Ali, Zaki Z. Sharawy

 

National Institute of Oceanography and Fisheries (NIOF), Aquaculture division, Cairo 11516, Egypt

Emanabbas03@yahoo.com

 



 Shrimp is the most important fishery traded on the world market in terms of present value. In many developing countries, this is the most profitable fishing trade . Shrimp fishing is not only a valuable food resource, but also an important economic resource in Egypt and  the other developing countries.  The  current research work is integrating DNA barcoding with morphology-based species identification as a valuable complementary tool. D ifferent  Egyptian  marine shrimp  species were morphologically described and characterized according to the shape of their  carapace; crest, groove and spines, the rostrum with its teeth; dorsally and ventrally, sex determination; the shape of petasma and thelycum , armed or unarmed telson and the body colour. The phylogenetic relationship of the shrimp species was studied using sequencing data from the GenBank database in part for the mitochondrial gene cytochrome oxidase (COI) subunit I subunit.  Similarity search was analyzed by using BLAST to compare the obtained sequences with their counterparts and revealed high similarity (≤ 99%) for with the compared sequences  in the GenBank database except for Trachypenaeus curvirostris (87% ) and Melicertus latisulcatus (88%) sequence identity . Among the studied sequences for the ten shrimp species from Egypt, the GC percent was ranged from 41.5% for Metapenaeus monoceros and 36.9% for Xiphopenaeus kroyeri .  Analysis of the  COI gene sequence for the phylogenetic tree divided the shrimp  species into two distinct clades that are genetically distinct from each other . This study is the first of its kind in Egypt as it provides both morphological and genetic clues for identifying such species in the Egyptian Mediterranean and Red Sea.