For many decades, shrimp aquaculture has relied on the culture of a few penaeid species, with a preference on Penaeus monodon and P. vannamei. These species have achieved such success that they are being raised outside their natural regions of distribution, raising concern about potential escapes and the ecological harm they could cause. As a result, the culture of native species is of considerable importance around the world, and significant efforts must be done in this area. Penaeus brasiliensis is a native species in the Yucatan peninsula with a great potential for culture. This species has been shown to thrive and survive at quite high densities and salinities above its isosmotic point (> 25 ppt). Given the karstic structure of the Yucatan peninsula’s ground and the saline intrusion tens of kilometers inland, inland culture of this native species could be a possibility. The aim of this work was to evaluate the suitability of the culture of P. brasiliensis in saline groundwater. Shrimp weighing 1.1 g were cultivated outdoors for 45 days in biofloc at 95 ind m2 in 3.14 m2 tanks (depth = 0.8 m) in triplicate in either saline groundwater collected from a well (60 m depth, salinity = 30 ppt) or natural seawater (salinity = 35 ppt). Shrimps were fed five times per day. Salinity was corrected with freshwater, aeration was provided with a 1 HP blower and diffusers, and each tank had an artificial substrate. Temperature and salinity decreased over time due to climatological conditions, which may be associated to a decrease in growth rate of shrimp at the end of the experiment. Nutrient levels (NH4, NO2, and NO3) were optimal for this species culture, and alkalinity decreased over time because of the nitrification process (no corrections were made). Shrimp performance was evaluated at the end of the experiment in terms of final weight, survival, growth rate, and final biomass. There were no significant differences in shrimp production between saline groundwater and natural seawater (Table 1), suggesting that inland shrimp culture is a possibility.