The current aquarium fish industry is a complex industry that links seedling farming to breeding-related supplies and breeding management and exhibition services, and the development of valuable and rare aquarium fish can generate huge profits. The mutagenesis mechanism and toxicity evaluation of the mutagenesis chemicals (ENU (N-ethyl-N-nitrosourea), Colchicine and Hydroxylamine) used in this study have been studied a lot, but studies on how mutagenesis chemicals affect zebrafish (Danio rerio) in physiological terms are insufficient. In this study, we analyzed the physiological changes of zebrafish exposed to mutagenesis chemicals.
The subject of the experiment was the second progeny (F2) of zebrafish treated with a mutagenesis chemical, with pH 6 to 7, photoperiod 10/14 h, about evaporation of the water in the system is exchanged with fresh water, and tetrabits were fed to the zebrafish twice a day. ENU, Colchicine, and Hydroxylamine were used as the experimental materials. In the experimental method, wild type of zebrafish was immersed in the experimental material at a certain concentration and time, and F1 was secured by crosslinking between objects immersed in the same material for 10 weeks, and F2 was secured.
After 10 weeks, the difference in expression genes was compared through the ΔΔCt method via Relative Quantification PCR for growth (GHra), immunity (Lysozyme) and nerves (NR4A2b) suitable for each tissue through sampling. Table 1.
In this study, the physiological change of Zebrafish and its progeny F1 treated with mutagenesis chemicals was compared and analyzed through the difference in genes expressing physiological changes in F2, the descendant of F1. In addition to the above genes, research and additional experiments on other genes involved in growth, immunity, and body color are needed.