There are several potential benefits associated with the use of a nursery phase in shrimp culture, such as stock inventory control, less predation, and greater uniformity of market-size shrimp post-larvae (PLs) at harvest. To optimize the use of energy and space, the density in nurseries should be maximized; although, high density can impact growth performance of the shrimp, survival rate and water quality parameters. Systems should also have environmentally friendly management practices that provide greater biosecurity to the culture system such as biofloc-type systems. Therefore, the objective of this study was to evaluate the effects of different stocking densities on the growth and survival of the Pacific white shrimp post-larvae under intensive nursery conditions in a biofloc system. A four-week nursery trial was conducted at the E.W. Shell Fisheries Center at Auburn University in Auburn, AL. The nursery system consisted of twenty-four 150 L polyethylene tanks recirculating with a sump (~ 800L) and circulation pump run as a common biofloc-type system. Eight different stocking densities were assigned as experimental treatments (0.5, 1, 2, 3, 4, 6, 8, and 10 PL/L). A sub-sample of PLs from each treatment (ie. tank from each treatment) was weighed every five days during the experimental period to determine when and if the growth diverged over time for shrimp reared at the various densities (Figure 1). At day 5, 10, and 15 of the experiment, it was observed that the growth of the PLs didn’t diverge between the different densities. At day 20 and 25, the growth of the PL diverged between the various densities. The results of the overall growth of the PLs throughout the trial were significantly different (P= <0.001) between the different densities. As it would be expected, the higher the stocking densities, the lower the growth of the PLs. In practical, a shrimp farmer would want to maximize the use of the nursery space to hold high stocking density, but at same time it’s important to find the appropriate stocking density to not inhibit the shrimp growth.