Vibrio parahaemolyticus is known as an infectious bacterium that is the lead deadly disease called “Human Acute Gastroenteritis” around the world. V. parahaemolyticus commonly has been identified and inhabited at the coastal waters in the United States and Canada, however, it has also been found in seafood products around the world in water and seafood. Detection of the virulent microbial strains like V. parahaemolyticus in seafood samples employing traditional culture techniques could be lengthy and requires traditional tools, thus alternative methods for rapid detection of pathogens is of great importance for food safety and disease diagnosis. In this regard, colorimetric detection can be effectively used as a sensitive, rapid, practical, and cost-effective diagnostic method.
The main objective of our study is to introduce and identify V. parahaemolyticus using a specific colorimetric detection through DNAzyme as a signaling probe. In our study, colorimetric-PCR based detection method has been used to identify V. parahaemolyticus using DNAzyme as a signaling probe. The DNAzyme HRPzyme sequence containing a G quadruplex nucleotide sequence has been used, which mimics peroxidase activity. The HRPzyme would then catalyze the oxidation of ABTS in the presence of Hemin as a catalysis substrate (Figure 1).
We expect the introduced colorimetric-PCR Based assay will be a specific and ultra-sensitive approach for the detection of V. parahaemolyticus from a wide verity of environmental samples.