Oyster aquaculture operations are intermittently plagued by mass summer mortality of both juvenile and adult Pacific oysters (Crassostrea gigas). These catastrophic mortality events can be marked by the loss of >50% of oysters, with some of the highest losses in juvenile oysters grown in ‘on-bottom’ cultures. Mass mortality events have become so common that they are now known collectively as Pacific Oyster Mortality Syndrome (POMS) events. POMS have been variably associated with elevated temperatures, eutrophication, low dissolved oxygen, and pathogen loads. This project explored the potential benefits of ‘priming’ oyster seed to acute, sublethal environmental stress – specifically, hypoxia and temperature – with the hope that this conditioning will enhance oyster survival during the subsequent commercial grow-out phase. Following conditioning oyster seed was allowed to recover for 2 months before repeat exposure to anoxic conditions. Our physiological results provide evidence that prior exposure to hypoxic conditions significantly increased survival of oyster seed to subsequent anoxia exposure. In addition, we have maintained individuals from these treatments on a commercial oyster farm in Morro Bay, California where we have monitored the long-term impact of these exposures on growth and survival across a summer growing season. Together our results may provide a new opportunity to condition Pacific oyster seed prior to out planting that may increase survival during hypoxic conditions.