This study was assessed the effects of dietary phytocee (herb mixture) combination with vit-C phosphate on the performance of white shrimp, penaeus vannamei, under density stress condition and disease resistance against Vibrio parahaemolyticus AHPND. The experiment focus on 2 factor; A) Vitamin C 0 and 1,000 ppm and B) Phytocee 1,000 and 2,000 ppm then factorial in CRD was assigned in 4 treatment diets and 6 replicates; a) diet with the sole Phytocee 1,000 ppm b) diet with the sole Phytocee 2,000 ppm c) diet with VIT-C PP 1,000 ppm and Phytocee 1,000 ppm, d) diet with VIT-C PP 1,000 ppm and Phytocee 2,000 ppm. Seven hundred and twenty juvenile shrimps with initial mean 1.4 gram/individual were randomly distributed into 240L of 24 glass aquarium that contained 120L of 15 ppt seawater and stocked 30 shrimp per aquarium (250 pcs/m3). Shrimp were fed experimental diets 3-8%BW, 4 times a day during 8 weeks of feeding trial. The growth performance and bath treatment of Vibrio parahaemolyticus AHPND 6.2X105 CFU/ml was investigated. The results showed that the growth performance in terms of shrimp production, final weight, average daily gain, and feed conversion ratio were significantly differences (p<0.05). In addition, the survival rate was not significantly differences (p>0.05). After Vibriosis AHPND immersion treatment, the survival rate was significantly high in group of shrimp fed diet with VIT-C PP 1,000 ppm and Phytocee 2,000 ppm follow by group of shrimp fed diet with the sole Phytocee 2,000 ppm and group of shrimp fed diet with VIT-C PP 1,000 ppm and Phytocee 1,000 ppm and lowest in group of shrimp fed diet with the sole Phytocee 1,000 ppm (p<0.05).
Therefore, Dietary phytocee 2,000 ppm combination with vitamin C phosphate 1,000 ppm was recommended for the advantage on growth performance and feed utilization of white shrimp, penaeus vannamei, under density stress condition of 250 pcs/m3 and benefit of high survival rate presenting on disease resistance against Vibrio parahaemolyticus AHPND immersion treatment. Moreover, the sole phytocee 2,000 ppm and dietary phytocee 1,000 ppm combination with vitamin C phosphate 1,000 ppm were recommended for economic feed to enhance growth performance, feed utilization and survival rate during Vibriosis AHPND challenge test.
Keywords: Phytocee, white shrimp, growth performance, survival rate, stress test, Vibriosis AHPND