Selecting high-quality post-larvae (PL) is very important for preventing diseases and achieving good growth in Litopenaeus vannamei shrimp farming. At the Hatchery level, microscopic tests were done to check the health and strength of the shrimp seed. More than 20 factors were examined, including the condition of the hepatopancreas, gut fullness, gill health, lipid levels, and ability to adapt to pond water (acclimation). Each factor was scored, and a total score of 90 out of 100 was set as the minimum passing mark by AquaExchange. Molecular testing using qPCR was also carried out to detect infections early. Between October 2024 and June 2025, this process was applied to 650 million seeds and achieved a 98.4% success rate. These results show that careful PL screening greatly reduces disease risk and improves overall farm performance.