Wheat subproducts have been widely used in shrimp diets as a carbohydrate source due to its quality and binding characteristics. Price volatility and supply issues have led researchers to explore other suitable cereals for animal feeds, such as barley. However, the main concern of barley use is the presence of non-starch polysaccharides (NSP), including (1→3;1→4)-β-D-glucan, also known as β-glucans, and arabinoxylans. These NSP may increase digesta viscosity, preventing nutrients from being digested. Dietary exogenous carbohydrases (enzymes that degrade β-glucans and Xylan) are commonly included in high fiber feed to enhance the disruption of cell walls from the NSP and release entrapped energy. In turn, this increases the available energy for maintenance and growth. Therefore, the current study evaluated the effect of dietary barley inclusion (as a wheat replacement) alongside carbohydrase supplementation on the growth performance and nutrient utilization of Pacific white shrimp. A 35-day growth trial was performed in a clear water recirculating aquaculture system. Six iso-nitrogenous and iso-lipidic diets (35 g 100 g-1 protein and 7 g 100 g-1 lipid) were made; no enzymatic addition was performed in two diets, which contained wheat (control) and barley as carbohydrate source, respectively. The four remaining diets contained only barley, two levels of xylanase (XYL), or a multi-enzymatic solution (MES). Shrimp were fed four times daily during this trial. Growth performance parameters such as survival rate, biomass gain, feed conversion ratio, and final average individual weight were analyzed, although no differences were observed (p>0.05). Subsequently, ten shrimp per replicate were preserved (-20 °C) for later whole-body proximate composition analysis and protein and phosphorus retention calculations. Additionally, gut and hepatopancreas tissues were collected to examine genes related to physiological function, the results of which will be shared in the presentation.