Hybrid catfish (I. punctatus female x I. furcatus male) farming industry is one of the top agriculture industries in Alabama and a priority for research and development. Hence there is a need to systematically look at feed formulations. This study investigated the effects of four plant protein sources, solvent-extracted- soybean meal (SBM), enzyme-treated soybean meal (ET-SBM), low oligosaccharide soybean meal (LO-SBM) and soy protein concentrate (SPC) in the diets on the growth performance, body composition and blood chemistry of juvenile hybrid catfish reared in a clear water system. Four ingredients were formulated into seven isonitrogenous (40%) and isolipidic (8%) test diets. A basal diet containing 50% SBM as the primary protein source. The SBM was incrementally replaced (50% or 100%) on an isonitrogenous basis with ET-SBM, LO-SBM or SPC. Fingerling hybrid catfish were stocked into 35 aquariums at a density of 20 fish/aquaria with mean weight of 4.29±0.07g (mean± s.d.) then fed with one of seven diets twice daily for eleven weeks. After the feeding trial no significant differences were found in weight gain(g), weight gain (%), feed conversion ratio (FCR), TGC, apparent net protein retention (ANPR) and survival rate between the hybrid catfish fed with the seven diets. there did seem to be a trend towards an increase in weight gain of fish fed with diets that solvent-extracted-soybean meal partially or fully replaced by those various processing soy protein sources. No significant differences or negative trends were found in the further studies in blood chemistry, hematocrit, hepatosomatic index (HSI) and intraperitoneal fat (IPF) value of those fish fed with seven experiment diets. In conclusion, it suggests that using ET-SBM, LO-SBM and SPC are three potential ideal ingredients which could partially or fully replace dietary solvent-extract soybean meal to feed with fingerling hybrid catfish.