Wild bonefish (Albula vulpes) were collected from two locations (Biscayne Bay and the Florida Keys) and transported to Harbor Branch Oceanographic Institute where they were acclimated to captive habitats to examine spawning mechanisms. The population underwent monthly hormonal manipulation to induce spawning. Spawning events occurred on October 5, 2020, and October 6, 2020. For both spawns, eggs were collected in an egg collector and transferred to Kreisel tanks to hatch. Samples of eggs were collected prior to hatching to determine biometrics such as size, fertilization success, and hatching success. After hatching, larvae were collected daily for up to 3 days post-hatch and biometric data (total length and oil droplet length) was recorded for a subsample of larvae. Total fatty acids (polar and neutral) were also extracted from egg and larvae samples and analyzed using GC-MS detection methods. Results are expected to show a decrease in essential fatty acids over time as they are utilized by larvae for growth and metabolic processes. This project is part of a multi-year effort to successfully spawn bonefish in captivity and will be utilized to develop suitable diets for captive larvae.