Global aquatic animal production has been increased, with a minimum impact on the environmental and benefit for human consumption. Aquaculture plays an important role in product of quality nutrition food; however, aquatic animals are susceptible to viral, bacterial, protozoan, parasite and other diseases . It is very important to realize that multiple pathogens are often present in the pond environment, and it is essential to consider the pathogen and the environment in order to prevent or treat disease problems before it becomes widespread. White Spot Disease (WSSV) has been the most problematic infectious agent . If a virus is present in a population and the environmental deteriorates, there will then be a rapid rise in mortality.
Our effort is of interest to shrimp disease diagnostics helping to improve healthy and safe aquaculture farm production. In our shrimp diagnostic work, DNA viruses (WSSV, Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV)) and RNA viruses ( Taura S yndrome V irus and Yellow Head Virus) can be detected using the PCR detection techniques to screen individual and pool of five samples. Here, we will share our wor k about the services on screening of a subset of samples sent for Genotyping for two pathogens WSSV and IHHNV with the estimation of their prevalence. It is possible to determine the prevalence with good sensitivity using DNA samples collected for genotyping services for qPCR testing. Table 1 shows a full comparison among lines and estimate minimum prevalence for each pathogen detection. The concentration of the viral load in the pooled samples (in number of copies/mL ) can be determined using qPCR with a standard curve . Among other applications, this technique is useful for monitoring pathogen prevalence in all-pathogen exposed breeding populations over time, or confirming groups of broodstock are free of detectable pathogen loads . Importantly, this service can be linked with genotyping work, providing a genetic profile and estimated viral load at reduced cost.