In this study , the low level of exposure was performed to assess the oxidative stress and biotransfo rmation of the glyphosate on Whiteleg shrimp Litopenaeus vannamei. The concentrations of herbicide as follow ing concentrations as Control(+),10ppm, 20ppm, 30ppm, 40ppm and 50ppm (Part per million concentration at 24hr durations (all are triplicate) . The samples were collected every 6hr time interval. Naturally, the aquatic organisms having self-defense mechanism come for rescue thus the elevated levels of different types of enzymes as a biomarker whether its elevation rate of hepatic cytochrome P450-associated enzyme activity to overcome the pollution risk. At the same time most possible the releasing of reactive oxygen species (ROS) rate is increased during the defense mechanisms occurred in the cell because of rapid oxidation, reduction, hydroxylation, dealkylation reactions . In the present activity of phase I reduction of three CYP450 isoforms (benzyloxyresorufin - O-dealkylase [BROD], ethoxyresorufin-O-dealkylase [EROD] and methoxyresorufin-O-dealkylase [MROD] ) enzymes were measured in the hepatic S9 fraction prepared from L. vannamei at different concentrations of glyphosate . The inducibility a nd activity of phase I reaction of three CYP450 isoforms (benzyloxyresorufin - O-dealkylase [BROD], ethoxyresorufin-O-dealkylase [EROD] and methoxyresorufin-O-dealkylase [MROD] ) enzymes were measured in the hepatic S9 fraction t reated with glyphosate at different concentrations in the laboratory condition within 24hr duration. The levels of BROD (CYP2B6), MROD (CYP1A2) and EROD (CYP1A1) in the tissues of L. vannamei were measured using Multimode reader . All the MFO enzymes exhibited a hierarchical dose-dependent activity in response to glyphosate concentrations. The level of superoxide dismutase (SOD), catalase (CAT) and Glutathone reductase (GR) in all the treatments decreased in a dose- and time-dependent manner except for the concentration 40-50ppm. Finally conclusion of these experiments revealed that 10-30ppm concentrations of glyphosate toward the normal condition when compared to other higher concentrations of 40ppm to 50ppm. The elevation level of increased both oxidative and biotransformation enzymes subsequently became normal at low level concentrations.