Atrazine is an herbicide used to prevent pre-emergence broadleaf weeds in crops such as maize, sugarcane etc.,. But these herbicides expert a different impact on exposed organisms, when it reached the adjoining land and water. Such paradoxical events could be avoided to preserve the bio-diversity of natural population. The atrazine exposed different concentrations ranges from 70 ppm, 80ppm, 90ppm and 100ppm (Parts per million i /e µg/L) at 40 days duration. In this study the biochemical assays of molecular biomarker development of Poecilia reticulata Gill, Liver, Gonad of fish were made against Glyphosate exposure. The specific activity of tissue damaging enzymes such as Glutamate Pyruvate Transaminase( GPT) and Glutamate Oxaloacetae Transaminase (GOT) and Alkaline Phosphattase (ALP) were also measured. Simultaneously, the antioxidant enzymes such as Catalase( CAT), Superoxide dismutase(SOD)and Glutathion reductase also measured. . This study comes to a conclusion that, the antioxidant and tissue damaging enzymatic activity was increasing at early stage, subsequently there is a gradual decrease d at the end of the exposures.Both antioxidant and tissues damaging enzymes was noted in P.reticulata at the lower concentration up to 70 ppm to 80ppm at 20 days duration whereas the 90-100ppm the elevation level of enzymes activity at 30days duration then declined their activities . However, GOT showed maximum activity was noted than GPT, and also reduction of GSI (Gonadosomatic index) as well as small changes in its HSI (Hepato somatic index) in all the concentrations. Biochemical and antioxidant enzymes response in fish can tolerated certain level of herbicide exposure at the low level concentrations.